Microbial Misadventures: Playing With Fire

By Rebecca Kreston | May 26, 2014 11:00 am

“Water-borne pathogen.” Three gut-twisting words with enough power to make any epidemiologist, public health official, or globetrotting tourist double over. One of the most common forms of disease transmission is the microbial hijacking of our most precious fluid. This mechanism of infection is employed by a motley crew of microscopic organisms that have adapted to prey upon our unquenchable thirst, from pervasive bacteria like cholera and typhoid to often less famous but no less formidable parasites such as giardia and dracunculiasis.

Oocysts of the cryptosporidium parasite stained magenta using the Ziehl-Nielson acid fast stain.

Oocysts of cryptosporidium stained using the Ziehl-Nielson stain. Image: Alae-eddine GATI. Click for source.

Yes, these pathogens commonly sidle their way to our coiling intestines via the ingestion of water, but often not via so prosaic a route as an icy glass of unpurified water or a nibble of some contaminated, unwashed greens. These microorganisms find their way to our gastrointestinal tract in ways as diverse as our assorted day-to-day interactions with water. One particular case associated with a little known parasite, Cryptosporidium parvum, illustrates how complex our interactions with H2O can be, and how innumerable and crafty are the routes by which pathogens frequently creep into our guts.

Cryptosporidium is a hardy protozoan parasite shaped like an irregular cannonball and is a puny little thing, topping out at 4 to 7 microns. It has a robust shell protecting it from environmental degradation, an armor so tough that it’s resistant to powerful disinfectants such as chlorination. This shield not only offers the parasite protection against unfriendly environments, but it also allows the parasite to survive for long periods of time outside the the guts of animals before cycling again through another animal with intestines.

Crypto, as it’s affectionately known by parasitologists, is spread in the fecal-oral fashion. The parasite begins its cycle via an animal’s ingestion of fecally contaminated water and goes on to infect and replicate within the epithelium of the microvilli of the small intestine. The disease, known as cryptosporidiosis, causes a watery diarrhea with abdominal cramps and nausea tossed in as a bonus. In some fortunate individuals the infection may be asymptomatic, but for most of us, the first symptoms of disease appear two to ten days after infection and the disease often runs its course for anywhere from a week to several weeks.

Crypto infection can cause significant morbidity in the immunocompromised. Indeed, it was only during the outbreak of the HIV/AIDS crisis in the 1980s that the parasite earned its street cred as a serious and ubiquitous pathogen.

The parasite is kind enough to restrict its shenanigans to the world of water. Outbreaks in humans typically revolve around recreational water exposure: swimming pools, water parks, water sprinklers, and wave pools. Crypto establishes itself in these popular summertime locales often through fecal contamination, either of the human or animal variety. Regarding the latter, runoff from farms and grazing pastures are often responsible.

Not content to simply spoil our summertime fun, crypto has proven quite adept at ruining even more mundane aquatic activities. Occasionally, crypto contaminates drinking water sources in rivers, lakes and wells, and this is where it has caused the greatest splash in terms of infections. It has caused humungous outbreaks affecting tens of thousands of people, most notably in the United States, Sweden and Ireland. The parasite made its big time break in Milwaukee, Wisconsin, in 1993 when it infected 403,000 people after one of the city’s two water purification plants were contaminated with runoff from cattle pastures (1).

In developing countries, crypto is responsible for 8 to 20% of all diarrheal disease and ranks among the top four of major pathogens causing diarrheal illness in children (2). Cryptosporidiosis is, of all the parasitic waterborne diseases, by far the most common and geographically expansive (2).

Until recently, crypto seemed so predictable, ruining our hot summer days spent in the tepid, toddler-filled waters of the local water park’s lazy river or casting a pall of fear over those of you fortunate enough to enjoy tap water that is not drawn directly from the cafe au lait-colored waters of the Mississippi. The effect of our limited understanding was that cryptosporidium’s role in an outbreak a few years ago was a surprise for those that thought that this little beastie was a one-trick pony.

In the early summer of 2011, a barn straddling the border of Indiana and Michigan caught fire. Firefighters from both states – three Michigan stations and one station from Indiana – responded to the scene and staged an incredible rescue, evacuating those at the greatest risk of harm: 240 calves born just that week. To extinguish the barn fire, “local hydrant water and onsite swimming pond water” were used (3). At the end of the day, though the barn was lost, the fire was extinguished and calves just embarking on the heydey (hayday?) of their cud-chewing lives were rescued. In other words, a success.

The number of probable and confirmed cases of cryptosporidiosis among the Michigan and Indiana firefighters that responded to the barn fire. Image: CDC. Click for source.

The number of probable and confirmed cases of cryptosporidiosis among the Michigan and Indiana firefighters that responded to the barn fire. Image: CDC. Click for source.

It was only a week later that the diarrhea hit and the Indiana station promptly reported an outbreak of gastrointestinal disease to the Indiana Department of Homeland Security. Of the 34 firefighters that responded to the fire, 20 fell ill and reported symptoms of diarrhea, abdominal cramps, nausea, vomiting, and fever. Several men missed workdays and one man was hospitalized. Firefighters from both companies were sickened, indicating that the source of their gastro ills likely originated from their joint firefighting effort.

Stool specimens from the firefighters yielded a curveball diagnosis: Cryptosporidium parvum. The parasite was also later isolated from the calves and the pond adjacent to the barn. An epidemiological investigation indicated that those firefighters that had direct calf contact, either carrying or maneuvering the calves from the barn to safety, had a higher incidence of infection (3).

But there were other likely exposures, though none found to be statistically significant or as cool as saving baby cows. The second-hand spray from hosing water to extinguish the fire may have contributed to the outbreak. Or it may have originated after the fire, when the men deglazed themselves of soot and ash with pond-water thereby infecting themselves inadvertently.

The Michigan health department suggested the farmer treat the well water and refrain from swimming in the pond. Meanwhile, the firetrucks, equipment and gear were also recommended to undergo a serious decontamination to be rid of residual crypto contamination

This is the first known instance of a cryptosporidiosis outbreak associated with a firefighting response. Yes, Cryptosporidium is a waterborne parasite but this particular outbreak is notable in that the affected population and the implicated activity of transmission were wildly different from what has been seen in the past. For public health officials, this outbreak neatly demonstrates the never-ending challenges of protecting the public from infectious diseases.

Of the many occupational hazards and flat-out dangers that firefighters are exposed to in their noble occupation, diarrhea is low on the ladder. Cardiovascular disease, hearing loss, and respiratory ills are the most common health problems among firefighters, not to mention the ever present dangers of battling blazing buildings and rescuing tree-bound felines (4). Now a wee protozoan parasite that causes diarrhea is a new and esoteric workplace hazard for firefighters

Fortunately for the rest of us, we rarely have to engage in such risky enterprises or other possibly hazardous aquatic activities such as drinking water, bathing, or floating down the lazy river.

References

(1) WR Mac Kenzie et al. (1994) A massive outbreak in Milwaukee of cryptosporidium infection transmitted through the public water supply. N Engl J Med. 331(3)161-7

(2) W Gatei et al. (July 2006) Cryptosporidiosis: prevalence, genotype analysis, and symptoms associated with infections in children in Kenya. Am J Trop Med Hyg75(1): 78-82

(3) Centers for Disease Control and Prevention (CDC). (2012) Outbreak of associated with a firefighting response – Indiana and Michigan, June 2011. MMWR Morb Mortal Wkly Rep. 61(9): 153-6

(4) JO Crawford and RA Graveling. (2012) Non-cancer occupational health risks in firefighters. Occup Med (Lond). 62(7): 485-95

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Body Horrors

Body Horrors looks at the history, anthropology and geography of infectious diseases and parasites.

About Rebecca Kreston

Rebecca Kreston is an infectious disease scholar trained in microbiology and epidemiology. She obtained her Biology degree from Reed College and her Masters of Science in Tropical Medicine from Tulane University. She's lived in tropical jungles, beaches and deserts around the world and has been exposed to several of the diseases that she studies. She currently lives in New Orleans, is a second year medical student and regularly battles insects of the Diptera, Siphonaptera and Hymenoptera orders.

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