The Lingering Mystery Of Why Humans – And Dogs – Yawn

(Credit: Fayzulin Serg/shutterstock)

(Credit: Fayzulin Serg/shutterstock)

A version of this article originally appeared on The Conversation.

You know the feeling. It’s impossible to resist. You just need to yawn.

A yawn consists of an extended gaping of the mouth followed by a more rapid closure. In mammals and birds, a long intake of breath and shorter exhale follows the gaping of the mouth, but in other species such as fish, amphibians and snakes there is no intake of breath.

But what’s behind a yawn, why does it occur?
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CATEGORIZED UNDER: Mind & Brain

Making Outer Space Smell Like Fresh Cut Grass

By John Wenz | July 5, 2018 4:59 pm
Scientists are bottling the smells of Earth so future astronauts can better dream of home. (Credit: NASA)

Scientists are bottling the smells of Earth so future astronauts can better dream of home. (Credit: NASA)

Nina Lanza expected Antarctica to be cold. After all, she and her seven fellow meteorite hunters weren’t allowed to board their transport in New Zealand until they’d proved they’d packed all the necessary gear. And she’d been warned about the endless daylight at their location smack dab in between McMurdo Station and the South Pole. But, as she says, “People try to tell you what it’s like, but it’s hard to describe because it’s so different from your everyday life.”
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CATEGORIZED UNDER: Space & Physics, Top Posts

An “Unholy” River Protects The Last Of These Rare Crocs

By Emily Sohn | July 4, 2018 8:00 am
Hatchling gharials from multiple females congregate near the dominant male — likely their father — on the territory where they hatched. (Credit: Dhritiman Mukherjee)

Hatchling gharials from multiple females congregate near the dominant male — likely their father — on the territory where they hatched. (Credit: Dhritiman Mukherjee)

Dead gharials began washing up on the banks of India’s Chambal River in December 2007. Over the following weeks, the body count grew. By mid-January, the dead reptiles—some the length of two tall men, lined up end to end—numbered in the dozens. By March, more than 110 of the skinny-snouted creatures had been found dead, most along a 30-kilometer (18-mile) stretch of river.

At the time, there were thought to be just 200 to 250 breeding-age gharials (Gavialis gangeticus) left in the world. And while only a dozen or so of the victims had reached reproductive age, many were close. A loss of more than 100 of them represented a major blow for a population already in crisis. Having been classified by the International Union for Conservation of Nature (IUCN) as critically endangered even before the die-off began, the species was clearly in trouble—including here on the Chambal, its last remaining stronghold.

Among the international team of veterinarians and crocodile experts that convened to investigate was Jeffrey Lang, a conservation biologist previously at the University of North Dakota. When Lang traveled to India that March, he was concerned about the die-off. But he was also driven by what he saw as a window of opportunity, opened by the crisis, to take a closer look at an understudied species. What he found were questions, loads of them—not just about what was killing gharials, but about how the animals lived in the first place.
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CATEGORIZED UNDER: Living World, Top Posts

Wannabe Space Pilgrims Test What Makes A Celestial Nation

By Cody Cottier | July 3, 2018 3:15 pm
(Credit: Dima Oris/shutterstock)

(Credit: Dima Oris/shutterstock)

When Igor Ashurbeyli walked onstage to be inaugurated Asgardia’s head of state, it was a few small steps for the Russian scientist and businessman, and one dubious leap for the world’s first “space nation.”

The national anthem that preceded him fills the mind with scenes of an epic, pioneering future, and that’s just what Ashurbeyli envisions. Our descendants take up residence in “space arks” with artificial gravity, a fleet of ships defends earthlings from asteroids and other “threats emanating from space.” Humans carry life to moon settlements and venture ever deeper into the universe.
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CATEGORIZED UNDER: Space & Physics, Top Posts

After 40 Years, NASA’s Voyager Probes Still Phone Home

By Doug Adler | July 3, 2018 1:30 am
This artist's concept depicts one of the Voyager spacecraft crossing the solar system's boundary into interstellar space. Though these spacecraft launched decades ago, many of their instruments remain functional. (Credit: NASA/JPL-Caltech)

This artist’s concept depicts one of the Voyager spacecraft crossing the solar system’s boundary into interstellar space. Though these spacecraft launched decades ago, many of their instruments remain functional.
(Credit: NASA/JPL-Caltech)

Launched in 1977, the unmanned space probes Voyager 1 and 2 carried out an extensive survey of the planets and moons of the outer solar system. Visiting Jupiter, Saturn, Uranus, and Neptune, the Voyager program can only be called an unqualified success on virtually every level. The twin spacecraft returned thousands of photographs and reams of scientific data that fundamentally changed our understanding of our entire solar system. Currently in interstellar space, Voyager 1 is the farthest man-made object from Earth. Voyager 2 is near the edge of our solar system and will one day also enter interstellar space.
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CATEGORIZED UNDER: Space & Physics

Larry David and the Game Theory of Anonymous Donations

By Brian Gallagher | June 20, 2018 3:51 pm
(Credit: s_bukley/Shutterstock)

(Credit: s_bukley/Shutterstock)

In a Curb Your Enthusiasm episode from 2007, Larry David and his wife Cheryl and their friends attend a ceremony to celebrate his public donation to the National Resources Defense Council, a non-profit environmental advocacy group. Little does he know that the actor Ted Danson, his arch-frenemy, also donated money, but anonymously. “Now it looks like I just did mine for the credit as opposed to Mr. Wonderful Anonymous,” David tells Cheryl. David feels upstaged, as if his public donation has been transformed from a generous gesture to an egotistical one. Cheryl says, about Danson, “Isn’t that great? He donated the whole wing. Didn’t want anybody to know.” “I didn’t need the world to know either!” David says. “Nobody told me I could be ‘anonymous’ and tell people!” He would have done it Danson’s way, he says, but, realizing the contradiction, he fumes, “You can’t have it halfway! You’re either anonymous, or you’re not.” What Danson did, David concludes, is “fake philanthropy and faux anonymity!” Read More

CATEGORIZED UNDER: Mind & Brain
MORE ABOUT: psychology

Organs Grown to Order

By Jeremy Rehm | June 19, 2018 3:04 pm
 Chimera: A genetically modified mouse embryo successfully grew a beating heart from rat stem cells. (Credit: Salk Institute)


Chimera: A genetically modified mouse embryo successfully grew a beating heart from rat stem cells. (Credit: Salk Institute)

More than 100,000 people in the United States need an organ transplant, but demand always outpaces supply. An average of 20 people in the nation died every day in 2016 because organs were unavailable, and that was despite record annual donations of more than 33,000.

Physicians have proposed many solutions to encourage organ donations, including payment. But scientists are looking elsewhere to ensure a better supply. Thanks to advances in genetic engineering, a new twist on using animals for transplants offers promise. Since the 1960s, a handful of patients have received an animal organ — xenotransplants of everything from a baboon liver to a whole chimpanzee heart. But many patients died because their immune system rejected the transplant.

Attempting to outwit evolution, Hiromitsu Nakauchi, a physician and geneticist at Stanford University, proposed a solution to this immunity problem: Instead of transplanting an animal’s organ, scientists could grow a customized human organ in an animal. By adding a patient’s own stem cells to an engineered animal embryo, Nakauchi and others hoped to grow a new, healthy organ ready for transplant.

Nakauchi was the first to demonstrate that the idea of growing one species’ organ in another species’ body was even possible, first between mice and rats in 2010. Another research group grew human cells in a pig in 2017, offering further proof of principle. Nakauchi recently reviewed his process in the Annual Review of Cell and Developmental Biology. We talked with him about this promising frontier as well as its many scientific, health and ethical challenges.

This conversation has been edited for length and clarity.

In your article, you talk about “mammalian chimeras.” What are those, and how do they relate to your research?

A chimera is an organism with cells from two genetically different individuals. The easiest chimera to understand is somebody with a heart transplant or bone marrow transplant: That patient has somebody else’s cells in their body. So they’re a chimera. My ultimate goal is to make human organs, and for that, we’re first trying to make animal chimeras.

Tell me a bit about that. How exactly does your idea work?

We’re basically mixing human stem cells with an animal embryo and some genetic engineering in between. The idea is that if we know how to control what genes code for, we should be able to generate organs from someone’s genetic blueprints.

About 25 years ago, I started working with hematopoietic stem cells, which are cells that can make all your blood cell types. At the time, they were the only stem cells we knew much about because they were used in bone marrow transplants. I still use them today. I thought if we studied and understood the mechanism of how those cells work, it may be possible to generate organs from a single cell using a patient’s own genetic blueprint.

In bone marrow transplants, the recipient must first have irradiation to remove his or her own hematopoietic stem cells. It’s called myeloablation, and without it, you really cannot ingraft stem cells. It’s like a hotel room: Unless the previous guests leave, and the room is empty, you can’t take any more guests.

So it cleans the slate to make room in the bone marrow for the new blood-forming cells?

Exactly. We still don’t know how it works, honestly, but without irradiation and emptying that so-called niche to create an open space, you really can’t ingraft donor stem cells. It’s quite amazing, and it’s a critical detail for my experiments.

If we just put stem cells into a normal animal embryo, our idea wouldn’t work because there’s no open niche in the embryo — it still has all the genes to make its own organ rather than the other individual’s. So the entire chimera would be a mix of cells from both individuals. But if we genetically modify the embryo so that it can’t make its own kidneys, for example, that will leave a space for the other individual’s genetic information to make the kidneys. That was the idea: Prepare a space for a certain organ in the animal embryo, combine the embryo with human stem cells to form a chimera, let it grow and you get an organ made of cells from the source you want — the patient.

Hiromitsu Nakauchi (Credit: Stanford University)

Hiromitsu Nakauchi (Credit: Stanford University)

In 2010, you implanted rat stem cells into a genetically engineered mouse embryo, and the mouse developed a rat’s pancreas. What did you learn from that experiment?

That was the initial experiment. The pancreas was functional, and those chimeric mice were happy, but the pancreas was mouse-sized; it was too small to transplant back to the rat. It showed organs can be generated in other species but couldn’t yet show they could be transplanted.

So we reversed the process in a later experiment. We injected mouse stem cells into rat embryos. This time we got a rat with a rat-sized mouse pancreas — a huge mouse pancreas. We couldn’t transplant the whole thing. But we could transplant islets, small clusters of cells that produce insulin in the pancreas. We do human-human islet transplants now to treat diabetes patients. We get those islets from people who’ve passed away, but again, the number of islets to transplant is very limited.

So that raises another question: Why can’t we just use organs from people who have died?

We don’t usually transplant from cadavers because the organ tends to be contaminated by bacteria immediately after death. You may know that if a patient is brain dead, heart transplant surgeons will take out the heart while it’s still beating. They don’t wait because the heart will start to deteriorate.

That’s a little shocking. So how do experiments with mice and rats lead to transplants for people?

Those early experiments provided evidence that a developing embryo can grow organs of other species. The organ was generated, useful and a perfect match. There’s no need for immunosuppression or fear of organ rejection because it’s the patient’s own organ. If this applies to humans and sheep or humans and pigs, we should be able to use a sheep embryo environment to grow human organs. Then cut it out and put it into the patient.

Isn’t that a long time to wait for an organ?

Pigs and sheep both grow very fast, so from our estimates, it would take about 10 months or less. It depends on the organ, though. For pancreas islets, it might require something like newborn piglets, whereas for something like a heart, you may have to wait longer.

What are the chances of a bacterial or viral infection?

Inside the animal body, it’s clean. Just like inside our abdomen, or in a uterus, or even in our bloodstream, it should be clean. Otherwise we’d get infected, right?

We do have specific pathogen-free pigs reared in a clean room that are regularly monitored for infections. So, that part is taken care of. Often brought up is the worry about pigs having an endogenous retrovirus, which some people say could activate and infect human cells. But that possibility has been almost denied. I’m not saying there’s no potential danger. There’s always a risk, and we’ll need a risk-benefit balance. But I consider a chance of getting some weird infection very small.

Editor’s note: Pig retroviruses called porcine endogenous retroviruses or PERVs are viruses that previously — maybe millions of years ago — infected pigs and left a bit of their viral DNA in all pigs. Studies have shown some of these viruses can reactivate in petri dish experiments using human cells, raising health concerns for xenotransplants. However, numerous studies looking at the threat of retroviruses infecting people have found no evidence of viral transmission.

What barriers or technical challenges still need to be solved?

A major barrier is doing these experiments in large animals — they’re not like mice and rats. They’re expensive, they can’t breed year-round, we can only use a limited number of them for experiments, and they’re evolutionarily distant from humans. But we now need to show we can generate organs in large animals. That’s the goal and the challenge, and we’ve still not succeeded. That’s my focus now. Once we’ve succeeded, then I’ll turn to the safety issues.

On a more technical side, gene editing technology has developed significantly over the past 10 years, which is great because otherwise using large animals would be impossible. We still need to optimize the culture conditions for human stem cells, though. When we use something like in vitro fertilization to make a sheep or pig embryo, we’d culture it for three or four days before embedding human stem cells. We still don’t know the best culture environment, however, and that’s causing reproducibility issues. We still need to develop that. Otherwise, this is pretty low-tech science.

Schematic shows how scientists might use animals to grow human organs.

If this sort of xenotransplant became practice, would we have animal farms meant specifically for transplants?

Probably not a farm, but we’d need a facility just like a farm. From the outside, it’s just a pig farm, but inside those pigs are human organs — your heart, your liver.

Aren’t there a lot of ethical issues with having farms meant just for harvesting organs? There’s something unsettling about that.

Yes, which is probably why the National Institutes of Health is not funding these studies, and Japanese companies follow government guidelines against making these chimeras. It’s an unpleasant feeling — an icky feeling. But we sacrifice maybe billions of pigs and sheep every year to eat, unless you’re vegetarian or vegan, which I’m sensitive to. If it’s OK to sacrifice animals for food, why not use a small part of those animals to save somebody’s life? I know many patients with a very low quality of life because of organ failures, diabetes, or complications from immunosuppressants after a transplant. Knowing these people as a medical doctor, I think this research is rational.

People have also talked about trying this in non-human primates. Is that really under consideration?

The idea is there, and I’m almost 100 percent certain we could make human organs in, say, a chimpanzee because we’re closely related. But it’s not easy because they’re not like mice and rats. They have very particular reproductive seasons and mate choices, it’s difficult to prepare a surrogate mother, and then it takes years for them to grow to a certain size.

And of course, there are the ethical questions. That’s an animal that looks somewhat human. That’s scary. I don’t like it. Unless it’s a kidney, which we could take one of, we’d have to sacrifice those chimps. But what are you going to do with a chimp with one kidney? We’d need to keep it, and that’s very expensive. So overall, it doesn’t really make sense. It’s not very practical.

How long until we might see this option for patients?

Five years ago, I thought we might be able to generate organs in pigs rather soon. But after doing some preliminary experiments, the genetic distance between these species and humans is too large. It’s not as simple as mice and rats, which diverged roughly 21 million years ago. But sheep and humans or pigs and humans separated about 94 million years ago. That makes it more challenging, and we don’t really know the factors hindering it; it could be a cell receptor, a ligand, cell adhesion molecules or something in the cell cycle. We don’t know.

How do people generally react to your work?

People initially have an icky feeling. But once I explain what we’re trying to do and the results we’re getting, they usually understand and become supportive, except for hardcore animal activists. I think people need to understand the benefits and potential risks of what we’re trying to do. Then people will understand better, and we’ll get more support, I hope.

Editor’s note: This story was updated March 25 to correct two points. The 2010 experiment by Hiromitsu Nakauchi involved induced pluripotent stem cells generated from rat skin cells, not pancreas cells, as the original story stated. Guidelines against developing chimeras in Japan were put in place by the government, not by companies.


This article originally appeared in Knowable Magazine, an independent journalistic endeavor from Annual Reviews. Sign up for the newsletter.

Knowable Magazine | Annual Reviews

CATEGORIZED UNDER: Health & Medicine

To Avoid Humans, More Wildlife Now Work the Night Shift

fox-london-night

An urban fox scavenges on the edge of a park. (Credit: Shutterstock)

For their first 100 million years on planet Earth, our mammal ancestors relied on the cover of darkness to escape their dinosaur predators and competitors. Only after the meteor-induced mass extinction of dinosaurs 66 million years ago could these nocturnal mammals explore the many wondrous opportunities available in the light of day.

Fast forward to the present, and the honeymoon in the sun may be over for mammals. They’re increasingly returning to the protection of night to avoid the Earth’s current terrifying super-predator: Homo sapiens. Read More

CATEGORIZED UNDER: Living World, Top Posts
MORE ABOUT: animals

How Can a Baby Have 3 Parents?

By Jennifer Barfield, Colorado State University | June 15, 2018 11:21 am
baby-3-parents

(Credit: Shutterstock)

It seems impossible, right? We have been taught from the time we were young that babies are made when a sperm and an egg come together, and the DNA from these two cells combine to make a unique individual with half the DNA from the mother and half from the father. So how can there be a third person involved in this process? Read More

When Does Hungry Become Hangry?

hangry-hungry-science

You’re ready to blow your top – but how much is due to your internal hunger and how much to external annoyances? (Credit: Shutterstock)

Have you ever been grumpy, only to realize that you’re hungry?

Many people feel more irritable, annoyed, or negative when hungry – an experience colloquially called being “hangry.” The idea that hunger affects our feelings and behaviors is widespread – from advertisements to memes and merchandise. But surprisingly little research investigates how feeling hungry transforms into feeling hangry. Read More

MORE ABOUT: psychology
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